The largest commission of Edward Steichen’s artistic career was his 1910–1914 In Exaltation of Flowers murals. These seven large-scale paintings debuted at the Knoedler Galleries, New York in 1915. After their deinstallation, the series was not shown together again for 102 years. The Art Bridges Foundation acquired the murals in 2016 and initiated a partnership with the Dallas Museum of Art (DMA). Research was carried out by the DMA curatorial team in preparation for the 2017–2021 traveling exhibition Edward Steichen: In Exaltation of Flowers. The DMA conservation team treated the paintings in a public-facing gallery and studied them with surface imaging techniques, non-destructive elemental analysis, and cross-section microscopy. Samples collected from two murals were later included in a multianalytical study at the Winterthur/University of Delaware Program in Art Conservation (WUDPAC). Research carried out at the DMA and WUDPAC has revealed Steichen’s choice of Lucien Lefebvre-Foinet canvases, modern oil paints, and gilding materials. Hidden away for over 100 years, the murals show a new facet of the artist’s oeuvre and how his technique evolved throughout the commission.